A83016f, a New Memberof the Aurodoxfamily1

growth-promotant activity in chicks6). As in the related compounds L-68 1 ,2177) and the phenelfamycins8), A83016F lacks the pyridone moiety commonin most members of this class. The strain A83016 also produces several kinamycin-type antibiotics including the novel compound A83016A9). This paper describes the isolation, structural elucidation, and antimicrobial activity of A83016F. Strain A8301 6 Fermentation Fermenter Inoculum Stock cultures of the producing organism were preserved in glycerol lactose (10% : 5%) solutions stored in the vapor phase of liquid nitrogen. Fermenter inoculum was prepared by transferring the culture into wide-mouth 250ml flasks containing 50ml of a mediumcomposed of glucose 1%, soluble starch 2%, yeast extract 0.5%, NZ-Amine A (Sheffield Products) 0.5% and CaCO3 0.1% in deionized water (pH 7.0 prior to sterilization). These flasks were incubated for 48hours at 30°C on a shaker